Microsoft Word - 36 张群英

Size: px

Start display at page:

Download "Microsoft Word - 36 张群英"

役乐
7 years ago
Views:

1 44 6 Vol.44, No OCEANOLOGIA ET LIMNOLOGIA SINICA Nov., 2013 (Pelodiscus sinensis)est-ssr * ( ; ; ; ) EST-SSR, 60 (Pelodiscus sinensis)-,, 15 EST-SSR, C8387 (P<0.05)(P<0.01), CC AB 4 ; C211 (P<0.01)(P<0.05), C, CC, BB AB ; C5670 (P<0.01)(P<0.05), BB AA ; C1312 C13038 (P<0.01), C1312 BC C13038 BB ; EST-SSR ; ; S941 (Pelodiscus sinensis),,,,,,,, (, 2005),, ( simple sequence repeat, SSR),, SSR(G-SSR) SSR(EST-SSR) EST-SSR,, G-SSR (Gupta et al, 2004; Skaala et al, 2004)Que (2007)Li (2010)Bu (2011) G-SSR,, EST-SSR (Zalapa et al, 2012), EST, QTL SSR (Zhang et al, 2012), (2013) EST, EST *, BE ;, PJ ,, @163.com :,, suda-shui@163.com : , :

2 6 : (Pelodiscus sinensis)est-ssr 1655 SSR,, EST-SSR EST,, EST-SSR,,, (Pelodiscus sinensis),,, 60,, 1.2 EST-SSR 15 EST,,, DNA 60,,, - DNA, (Thermo Fisher) DNA, 100ng/L, L PCR, 2ES TaqMIX 10L, (10mol/L) 1L, DNA 1L, 20L, PCR (TAKARA), : 94 2min, 26 (94 30s, s, 72 30s), 72 2min6%, (2009), Gel-Pro Analyzer (1) Tab.1 表 1 微卫星位点及引物信息 Information of the microsatellite loci and primers sequence (5 3 ) NR-tophit_description C8433 (AT) 9 C8387 (ATTT) 4 C4663 (GT) 8 C211 (CGG) 5 C5670 (AGG) 5 C5960 (AGC) 4 C0009 (GT) 9 C8513 (GT) 9 C11010 (CCTT) 4 C25692 (GT) 9 C2226 (CT) 10 C4950 (AC) 11 C1312 (CT) 11 C13038 (CCT) 5 C1675 (GT) 11 F: ATGCCAGCGTTGTGCTTTC R: CAAATCTGCCCTACGTCGC F: GCTGTTCCGACCCAGTGTC R: AGCACCTGTAGGAGGACTTG F: AAACAACAGGATTTCCATGCTC R: CCTCCCTCAGACAAGCTCC F: AGTTCCCTGAGCAGTACCC R: ACCTGCTTACAGCTGAGTTTC F: CAACGGATGCACTCTCGC R: AGTGTTAGGGACAGCGCC F: CTGCATCCTTGGCTTCGTG R: CTGCATCCTTGCGTTCGTG F: CATCGCAACCTTGGAGAGAC R: CTGGCTAATTGTGCAGACCG F: CCAATGCGAATGAGTTTGCC R: CTGGCTAATTGTGCAGACCG F: AGCTGGGCAATAGATGGCTG R: ATGAGGCAGACAAGAGCC F: ATCAAACCTCCAGGGCTCC R: GAGATTGTCCCTGGTTGGC F: GGCACAGTTTGATCAGTGGG R: GGGTGAAGACTCTGATGGG F: GCTTGCATAGATCCGGCAC R: TGATACCTAGGACCGCAGG F: AGAGGATTGACCTAAGAAATTG R: AGGGAACTGAGCAAGGGTG F: GATGCAGCGAAGGAACACG R: GGGCTTTGGTGACGACTTG F: CAAGAAGTCCGGCTTTGGC R: CTCCAGAGATATGCCTTCCAG NR-tophit_description: NR E3A[] B [] G 3 [] 2D [] Der1-like 1 [] RNA RPB1 []

3 , Gel-Pro Analyzer 4.5 PopGen32 (P)(N a ) (N e )(H o )(H e ), Botstein (1980) PIC (2) SPSS 16.0 Shapiro-Willk 4, (GLM) 15 SSR 4, ANOVA, , 60, 4 (, 2011) SPSS 16.0 Kolmogorov-Smirnov, P 0.05, 4 ( 2), 2.2 PCR, 15,,, 1 2 C8433 C0009 表 2 中华鳖的体重体长背甲长裙边宽正态分布检验 Tab.2 The test of normal distribution in body weight, body length, carapace length and side width of P. sinensis P , (N a ), (N e ) (H o )(H e )(PIC) ( 3) ,, 5, C8387 (P=0.021)(P=0.02) (P=0.032)(P<0.05), (P=0.0087) (P<0.01); C211 (P=0.007) (P=0.009) (P<0.01), (P= 0.028)(P<0.05); C5670 (P=0.000) (P<0.01), (P=0.042) (P<0.05); C1312 C13038 (P=0.009) (P=0.002)(P<0.01) 5, ( 4): C8387, CC 4, AB (P<0.05), CC 4, AB C5670, BB Fig.1 1 C8433 PCR amplification result of C8433 loci in P. sinensis Fig.2 2 C0009 PCR amplification result of C0009 loci in P. sinensis

4 6 : (Pelodiscus sinensis)est-ssr 1657 表 3 Tab.3 中华鳖群体 15 个微卫星位点的遗传多样性统计 Data of genetic diversity of 15 microsatellite loci for P. sinensis N a N e H o H e PIC C C C C C C C C C C C C C C C N a, N e, H o, H e, PIC AA (P<0.05), BB, AA C211 B,, CC AB AA BB (P<0.05), BC AB (P<0.05), ABAABB (P>0.05), C ; CC (P<0.05), 4 (P>0.05); CC, BB (P<0.05), BBBCABAA (P>0.05), CC ; BB AB C1312 Der1-like, 4 表 4 中华鳖 5 个微卫星位点不同基因型体重体长背甲长和裙边宽的平均值及多重比较 Tab.4 Means and multiple comparisons of body weight, body length, carapace length and side width of P. sinensis in 5 microsatellite loci (g) (cm) (cm) (cm) C8387 AB a a a a AA b ab ab ab BC b b ab b CC b b b b C5670 AA a a a a AB b a a a BB b a b a C211 CC a a a a BC ab a b ab AA bc a b ab BB bc a b b AB c a b ab C1312 AB a a a a BB ab a a a AA b a a a BC b a a a C13038 AB a a a a AA a a ab a BB a a b a (P<0.05)

5 , AB BC AA (P<0.05), 3 (P>0.05) AB,, BC, C13038 RNA RPB1,, BB, AB, BB , , ,, (, 2011),, (, 2010)(H o ) , (H e )0.5961,,,,,, (PIC), 15, 6 (0.25<PIC<0.5), 9 (PIC>0.5), 15 PIC , (PIC>0.5) EST EST-SSR,, 3.2,,,,, 3, -(, 2010) 15 SSR, C8387, ; C211, ; C5670,, C1312 C13038 ; SSR,, (, 2002;, 2011),,, (, 2012), (2013), (P<0.01), C211 C1312 C13038 EST,, ---,,, (, 2009)(, 2010;, 2013) (, 2004;, 2011) (Wang et al, 2013), EST EST-SSR,, EST-SSR QTL, 4 15 EST-SSR,, : (1) 15 (N e )(H o )(H e ) (PIC) , (2) 15, 5 : C8387 (P<0.01), (P<0.05), C211 (P<0.01), (P<0.05), C5670 (P< 0.01), (P<0.05), C1312 C13038 (P<0.01) (3) C8387 CC 4

6 6 : (Pelodiscus sinensis)est-ssr 1659, AB ; C5670 BB, AA ; C211 C, CC, BB AB ; C1312 BC C13038 BB,,, 鲀., 41(5): 9 16,,, :, 39(2): ,,, (Pelodiscus sinensis) (Morganella morganii)., 44(3): , , 33(5): ,,, RAPD., 28(2): ,,, , 34(2): ,,, , 36(005): ,,, , 17(1): 1518,,, :, 7879,,, QTL., 28(4): ,,, , 35(2): ,,, F 1, F 2 F 3., 20(2): ,,, 2011.,, QTL., 33(12): ,,, , 17(4): ,,, PAGE., 31(6): ,,, 2005.,. :, 18(2): Botstein D, White R L, Skolnick M et al, Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32(3): 314 Bu X J, Liu L, Wang L et al, Isolation and characterization of 21 novel polymorphic microsatellite loci in the Chinese soft-shelled turtle Pelodiscus sinensis. Genetics and Molecular Research, 10(2): Gupta P K, Rustgi S, Molecular markers from the transcribed/expressed region of the genome in higher plants. Functional & Integrative Genomics, 4(3): Li Z Q, Li J L, Feng X Y et al, Sixteen polymorphic microsatellites for breeding of Chinese soft-shelled turtles (Pelodiscus sinensis). Animal Genetics, 41(4): Que Y, Zhu B, Rosenthal H et al, Isolation and characterization of microsatellites in Chinese soft-shelled turtle, Pelodiscus sinensis. Molecular Ecology Notes, 7(6): Skaala Ø, Høyim B, Glover K et al, Microsatellite analysis in domesticated and wild Atlantic salmon (Salmo salar L.): allelic diversity and identification of individuals. Aquaculture, 240(1): Wang W, Li C, Ge C et al, De-novo characterization of the soft-shelled turtle Pelodiscus sinensis transcriptome using Illumina RNA-Seq technology. Journal of Zhejiang University Science B, 14(1): 5867 Zalapa J E, Cuevas H, Zhu H et al, Using next-generation sequencing approaches to isolate simple sequence repeat (SSR) loci in the plant sciences. American Journal of Botany, 99(2): Zhang H, Wei L, Miao H et al, Development and validation of genic-ssr markers in sesame by RNA-seq. BMC Genomics, 13(1): 316

7 CORRELATION OF EST-SSR MARKERS WITH GROWTH TRAITS IN CHINESE SOFT-SHELLED TURTLES (PELODISCUS SINENSIS) ZHANG Qun-Ying 1, HUANG He-Zhong 1, YUAN Wen-Cheng 1, LI Wen-Long 1, YONG Fu-He 2, XU Xue-Ying 3, ZHANG Ping 4 (1. School of Medicine and Life Sciences, Medical College of Soochow University, Fisheries Research Institute of Soochow University, Suzhou, ; 2. Mashe Special Aquaculture Farms in Xukou Town, Wuzhong District, Suzhou City, Jiangsu Province, Suzhou, ; 3. Fisheries Technology Extension Station of Suzhou City, Jiangsu Province, Suzhou, ; 4. Fisheries Technology Guide Station of Suining County in Xuzhou City, Jiangsu Province, Xuzhou, ) Abstract 15 polymorphic microsatellites were developed from the transcriptome sequencing EST database of Chinese soft-shelled turtles (Pelodiscus sinensis) to analyze the genetic diversity and linkage of 60 individuals. The result indicate that: 15 EST-SSR markers obtained amplified products successfully in a high polymorphism level; the number of mean valid alleles was ; the value of mean expected heterozygosity was ; and the mean polymorphism information content (PIC) was The linkage analysis showed the locus C8387 had a significant impact on body weight, body length, carapace length and a extremely significant difference in side width (P<0.01), and CC at C8387 was a favorable genotype for the four growth traits while AB was a disadvantage genotype; locus C211 showed an extremely significant impact on body weight, carapace length (P<0.01) and a significant impact on the side width (P<0.05), among them allele C was negatively associated with the body weight and genotype CC was disadvantage genotype of carapace length and side width, while BB and AB were favorable genotypes of side width and body weight respectively; locus C5670 had an extremely significant impact on body weight (P<0.01) and a significant impact on carapace length (P<0.05), in which BB was the dominant genotype of body weight and carapace length while the AA was the negative genotype; the locus C1312 and C13038 had a extremely significant correlation of body weight, carapace length, respectively (P<0.01), in which BC of locus C1312 and BB of C13038 was dominant genotype of body weight and carapace length, respectively. Key words Pelodiscus sinensis; EST-SSR marker; growth trait; correlation analysis

Microsoft Word - 2-曾聪（new）.doc

Microsoft Word - 2-曾聪（new）.doc 36 6 Vol.36, No.6 2012 6 JOURNAL OF FISHERIES OF CHINA June, 2012 文章编号 : 1000-0615(2012)06-0809-06 DOI:10.3724/SP.J.1231.2012.27809 团头鲂 3 个地理种群杂交效果的配合力和微卫星标记预测 1, 1, 1, 1, 1, 1,