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1 理 CNIC 行 95 年 年 林 96 年 02 27

2 料 來 理 (1) (2) (3) 率 立 了 率 不 不 率 DPPH 力 TEAC OH 率 老 行 2 年 7% 度 GDP 率 兩 來 (1) 力 類 來 (2) 力量 行 略 (3) 理 行 - 1 -

3 來 老 行 老 年 領 類 利 類 老 流行 金 都 都 類 降 罹 率 不 了 率 - 2 -

4 留 率不 年 了 利 不 A. 1 DPPH 力 DPPH 藍 力 DPPH 來 力 若 DPPH DPPH 行連 DPPH 力 DPPH 力 不 度 利 度 (ELISA reader) OD 540 nm - 3 -

5 IC 50 力 DPPH 力 ( ) = [1- A540 nm, sample/a540nm, blank)] TEAC 力 ABTS ABTS. + 離 藍綠 若 ABTS. + 離 力 降 力 trolox TEAC 力 力 力 不 度 利 度 OD 734 nm IC 50 力 力 ( ) = [1-(A734 nm, sample / A734 nm, blank)]

6 3 冷 力 I II III IV (a) (b) (c) (d) (e) (f) (g) 50µL I 量 1 ml II 量 1.6 ml III 量 100µL IV 量 量 數 量 冷 冷 數 10µL 冷 (h) 冷 量 冷 度 降 數 - 5 -

7 冷 ( A A intial - A sample ) 冷 (A intial ) 論 DPPH system TEAC system 力 DPPH system 度 µg/ml CF GR RR 率 85.6 % 83.9% 51.6% TEAC system 度 0.04 µg/ml GR CF TH 率 94.4 % 80.9% 76.0%( 1) DPPH system TEAC system GR CF 力 ME 力 GR CF sample sample - 6 -

8 離 行 力 Trolox EGCG Ascorbic acid 1. 力 度 率 DPPH system µg/ml CF 85.6% GR 83.9% RR 51.6% TEAC system 0.04 µg/ml GR 94.4% CF 80.9% TH 76.0% 力 力 2 DPPH system HGR AGR EGCG 力 ACF Trolox 力 HME AME 力 TEAC system 類 來說 EGCG 力 HGR AGR EGCG 力 ACF 不論 DPPH system - 7 -

9 TEAC system 力 HCF 來 力 Trolox 2. DPPH system TEAC system IC 50 (ppm) DPPH system TEAC system HGR AGR HCF ACF HME AME Trolox EGCG Ascorbic acid HGR AGR OH 力 EGCG Ascorbic acid 力 HCF Trolox OH 力 HME AME OH - 力 O 2 Trolox 數 參 H 2 O 2 力 HGR ACF HME 力 HCF AME 力 - 8 -

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