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1 ( ) Journal of Hunan Agricultural University (Natural Sciences), Aug. 2015, 41(4): DOI: /j.cnki.jhau 异樟叶精油及其主成分含量的动态变化研究 1,2 1* 1 ( ) 摘要 ,8 α ,8 1 3 α 关键词中图分类号 S 文献标志码 :A 文章编号 : (2015) Dynamic changes of essential oil and main components from isonerolidol type leaves Hu Wenjie 1,2, Jiang Xiangmei 1*, Yang Haikuan 1 (1.Camphor Engineering and Technology Research Center for State Forestry Bureau, Jiangxi Forestry Academy, Nanchang , China; 2.College of Life Sciences of Jinggangshan University, Ji an, Jiangxi , China) Abstract: In order to raise utilization ratio of the essential oil and main components from isonerolidol type leaves, and determine the optimum harvesting time of leaves, dynamic change rule of the essential oil and main components contents during different months from isonerolidol type leaves were tested and analyzed. Results indicated that there were significant differences in the average of oil content in isonerolidol type leaves from different months, the variation trend of average oil yield in isonerolidol type leaves was gradually ascending from March to September, then gradually declining till the next growth period of January. Therefore, September was the best harvesting time for the oil; However, the contents of isonerolidol, 1,8 cineole, α terpineol, methyl eugenol, methyl isoeugenol,sabinen, and 3 methyl 2 butenoicacid cyclobutyl ester in the oil of isonerolidol type leaves were significant difference between the different months, except for spatulenol and caryophyllene. The best harvesting time for isonerolidol, 3 Methyl 2 Butenoicacid, cyclobutyl ester, 1,8 cineole, methyl isoeugenol, α terpineol, methyl eugenol and sabinen was from May to July, in November, in January, in March, in May, in January and March, respectively. Keywords: isonerolidol type; leaf essential oil; main component; harvesting time (Cinnamum camphora (L.)Presl.) (Lauraceae) 收稿日期 : 修回日期 : 基金项目 ( ) (2010AZX00500) 作者简介 (1974 ) huwenjie2008@126.com * Xiangmeijiang@yahoo.com.cn

2 408 ( ) ,8 [1] [2 3] ( ) ( ) ( ) () ( )5 [4] [5] [5 7] 1 材料与方法 1.1 供试异樟样品的采集 ( ) 500 g 1.3 叶精油的提取 200 g ( 65 min ml g W ) ( ) 1.4 叶精油主成分含量的检测 Perkin Elmer Clarus 680 Perkin Elmer Clarus 600C Elite 5 MS 30 m 0.25 mm 0.25 μm min 3 /min min 15 /min min 53 min He 1.0 ml/min 0.5 μl 10 1EI MS EI (m/z) 质谱检索及数据处理 Nist SPSS (%) arcsin x 2 结果与分析 2.1 异樟叶平均含油量的动态变化 (0.51%) (1 ) 1.89 ( 2) (P ) 表 1 异樟叶不同月份的含油量 Table 1 The oil content from isonerolidol type leaves in different months /% /% F 0.05 F ± a A ± b B ± c C ± d D ± e E ± f F 表 2 异樟不同月份叶含油量的方差分析结果 Table 2 Result of variance analysis of oil content from isonerolidol type leaves in different months F P

3 异樟叶精油主成分相对含量的动态变化 GC MS 9 6 ( 1%) 2 1,8 α 9 ( 3) (41.13%) (24.39%) 1 3 1, (14.93%) 7 11 α (2.09%) (1.54%) 表 3 异樟叶精油主成分含量不同生长期差异显著性分析 Table 3 Significant analysis of main components contents of isonerolidol type leaves oil during the growing periods /% /% F 0.05 F 0.01 /% /% F 0.05 F ± a A ± a A ± a A ± ab AB ± ab AB ± ab ABC ± bc AB ± bc BC ± c B ± bc BC ± c B ± c C ± a A ± a A ± ab A ± b B ± b A ± bc B ± c B ± bcd B ± c B ± cd B ± c B ± d B 1, ± a A ± a A ± b B ± ab A ± c BC ± ab A ± cd CD ± ab A ± de CD ± ab A ± e D ± b A ± a A ± a A ± ab AB ± ab A ± abc AB ± ab A ± bc AB ± ab A ± bc AB ± ab A ± c B ± b A α ± a A ± ab AB ± abc AB ± bcd B ± cd B ± d B

4 410 ( ) ( 4) 7 (41.13%) 5 (41.07%) (24.39%) 9 (22.80%) ,8 1 (14.93%) 1,8 1 3 (10.68%) 3 α 3 5% α 5 (2.09%) 1 (1.54%) 3 (2.41%) α 表 4 异樟叶精油主成分相对含量生长期方差分析 Table 4 Variance analysis of main components contents of isonerolidol type leaves oil during the growing periods F P , α 结论与讨论 (0.51%) 1 [3] [3]

5 [8] [9] [10] [11] [12] 9 9 [13 15] 5 7 α [3] 参考文献 : [1] Pino J A Fuentes V Leaf oil of Cinnamomum camphora (L.)J Presl from Cuba[J] Journal of Essent oil Research (5) [2] [D]2006 [3] [D] 2006 [4] [J] (3) [5] [J] (6) [6] [J] (11) [7] [J] (2) [8] [D] 2013 [9] Croteaur Biosynthesis and catabolism of monoterpenoids [J] Chemical Reviews [10] Banthorpe D V Ekundayo O Mann J et al Biosynthesis of monoterpenes from 14 C labelled acetate and CO 2 [J]. Pytochemistry [11] Salisbury F B Ross C Plant Pysiology[M] Delhi Prentice-Hall of India Private Limited 1974 [12] Rajeswara Rao B R Bhattacharya A K Kaul P N et al. Changes in profiles of essential oils of rose-seented geranium(pelargonium sp.) during leaf ontogeny[j]. Journal of Essential Oil Research 1993(5) [13] Yamaura T Tanaka X tabata M Lighat dependent formation of glanular trichomes and monoterpenes in thyme seedling[j] Phytochemistry [14] Voirin B brun N Bayet C Effests of day length on the monoterpene composition of leaves of Menthaхpiperta[J]. Phytochemistry [15] [J] (4) 责任编辑 : 尹小红英文编辑 : 梁和

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