SP SS ATP MLCK c- kit MTL SP SS P < 0.05P < 0.01 ATP MLCK c- kit P < 0.05P < 0.01 P < 0.05P < 0.01 R285.5 A

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1 186 Traditional Chinese Drug Research & Clinical Pharmacology2015 MarchVol. 26 No. 2 MsPGN TGF- β 1/Smads/ILK TGF- β 1 Smad3 ILK Smad7 mrna MsPGN TGF- β 1/Smads/ ILK β β β β β SD 10.0 g kg g kg mg kg d 1 10 d 10 d MTL P

2 SP SS ATP MLCK c- kit MTL SP SS P < 0.05P < 0.01 ATP MLCK c- kit P < 0.05P < 0.01 P < 0.05P < 0.01 R285.5 A d o i /j.issn E ffect of Raw or Bran-fried Rhizoma Atractylodis on Gastrointestinal Motility of Experimental Spleen Deficiency Rats LIU Fen 1 2 LIU Yanju 2 TIAN Chunman1. 1 Hubei Institute for NationalitiesEnshi HubeiChina2. Hubei University of Chinese MedicineWuhan Hubei China AbstractObjective To observe the effect of raw or bran-fried Rhizoma Atractylodis RAon gastrointestinal motility of rats with experimental spleen deficiency syndromeand to explore the possible mechanism. Methods Fifty male SD rats were evenly randomized into normal groupmodel groupraw RA 10.0 g kg -1 group bran-fried RA 10.0 g kg -1 groupand domperidone5.0 mg kg -1 group. Except for the normal groupthe rats in other groups were fed with Xiaochengqi Decoction plus irregular diet to induce spleen deficiency syndromeand the modeling time covered 15 d. After the establishment of modelthe medication groups were given the corresponding medicineand the normal group and model group were given same volume of saline once a day for 10 days. After treatmentgastric emptying rate and intestinal propulsion proportion were evaluated after gastric gavage of Carbo Medicinalisblood was sampled from abdominal aortic to detect rat plasma motilin MTL substance PSP and somatostatinsswith radioimmunity. Colorimetric assay was used for the determination of ATP content of rat jejunum epithelial cellsand immunohistochemical method was used to detect the expression of myosin light chain kinasemlckin rat gastric antrum smooth muscle cells and c-kit in colonic tissues. Results Compared with the model groupthe gastric emptying rate was decreased and intestinal propulsion proportion was increased significantly in the normal groupraw RA groupbran-fried RA groupand domperidone groupwhile the plasma MTLSP and SS contents were increased to different degreesand ATP content of jejunum epithelial cellsmlck expression of rat gastric antrum smooth muscle cells and c-kit expression of colonic tissues were also increasedthe differences being statistically significantp < 0.05P < Compared with raw RA group bran-fried RA group had more obvious effectand the differences between the two groups were statistically significantp < 0.05P < Conclusion RA extract can exerts an overall improvement of rat gastrointestinal dysfunction induced by spleen deficiencyand the effect of bran-fried RA is superior to that of raw RA group. KeywordsRhizoma Atractylodis extract Fried with branspleen deficiency syndromegastrointestinal function Signal transduction

3 188 Traditional Chinese Drug Research & Clinical Pharmacology2015 MarchVol. 26 No g kg g kg [1] mg kg [2] [3] g kg d d 1.1 SD ± g SKSF % 2 % CMC- Na 2.5 ml 500 g 30 min 1 % % ml 6 ml 12 h 80 % 3 2 h g L - 1 %= % CMC- Na [I 125 ] MTL PSP SS EDTA- 2Na h - / 100 % ATP MTL P SP smmlck Cajal SS MTL SP c- kit SP DAB SS ATP cm 0.5 cm 1. 3 GC γ ATP UN DG5032 MLCK c- kit UT mm 0.8~ 1 cm XSP- 1 4 % SABC Image- Pro Plus 5.1 Image- Pro Plus 5.1 Motic MLCK

4 c- kit 5 ATP 5 P < MLCK c- kit 2 M T L S P S S x ± s n = SPSS17.0 Table 2 Effect of Atractylodes rhizome extract before and after ± x ± s Fried with Bran on Plasma MTLSP and SS content of model rat P < ± ± 0.01 P < P < 0.05P < P < 0.01 P > 0.05 P < A T P x ± s n = 1 0 Table 3 Comparison of ATP content in the jejunum tissue of rats P < in different groups 1 x ± s n = 10 Table 1 Comparison of gastric emptying and intestinal propulsion of rats in different groups ± ± ± ± ± ± ± ± ± ± ## P < 0.01 * P < 0.05 ** P < 0.01 P < 0.05 P < 2. 2 M T L S P S S MTL SP SS MLCK c- kit P < 0.01 P < 0.01 MLCK MTL SP SS c- kit P < 0.05P < 0.01 P < 0.05P < 0.01 MLCK c- kit SP P < 0.05 MTL SP P < 0.05P < 0.01 c- kit SS P < 0.05P < 0.01 P < 0.05 MTL SP SS MLCK c- kit P < 0.05 P < 0.05P < A T P ATP P < ± ± ± ± ± ± ± ± ± ± ± ± ± ## P < 0.01 * P < 0.05 ** P < 0.01 P < 0.05 P < 0.01 μ ± ± ± ± ± ## P < 0.01 * P < 0.05 ** P < 0.01 P < 0.05 P < 0.01 P < M L C K c - k i t MLCK c- kit ATP P < 0.05P < 0.01 ATP P < 0.05P < 0.01

5 190 Traditional Chinese Drug Research Clinical Pharmacology 2015 March Vol 26 No 2 A. 正常组图1 B. 模型组 C. 多潘立酮组 D. 生苍术组 E. 麸炒苍术组大鼠胃窦平滑肌细胞内 M L C K 免疫组化染色图 200 Figure 1 The inimmunohistochemical staining results of MLCK in gastric antrum smooth muscle cells of rats 200 A. 正常组图2 B. 模型组 C. 多潘立酮组 D. 生苍术组 E. 麸炒苍术组大鼠结肠 c - k i t 免疫组化染色图 200 Figure 2 The inimmunohistochemical staining results of colonic c- kit of rats 200 表4 对大鼠胃窦平滑肌细胞内 M LC K 结肠组织中 c - k i t 表达量的影响 x ± s n=10 Table 4 Comparison of the expression of MLCK in gastric antrum smooth muscle cells and c- kit in colon tissue of rats in different groups 剂量ｇｋｇ１ＭＬＣＫｃｋｉｔ正常组１１０２±３４９１６２７±４０１模型组组别生苍术组１００麸炒苍术组１００多潘立酮组０００５５０７±１２９９０５±２６４１０９９±３１７９１４±２７７功能低下性疾病都存在脾虚证的证候运用补脾运脾法多能奏效因此临床上对脾虚证的治疗其重要法则就是调理脾胃脾胃功能与胃肠运动关系密切胃肠运动对食物的消化和吸收意义重大目前文献记载的制备脾虚证模型的方法很多如情志失和法苦寒泻下法过度疲倦法饮食失节法 [6]等根据李时５６９±１２１珍的论述大黄因其性苦寒能耗伤元气阴血损伤１２０７±２８７脾胃因此在大黄苦寒泻下基础上改进的破气苦降１５９８±３８１加饥饱失常法是目前较普遍运用的一种建模方法且９０１±２３２注与正常组比较 ##P 0.01 与模型组比较 *P 0.05 **P 0.01 与多潘立酮组比较 P 0.05 P 0.01 与生苍术组比较 P 0.05 简单易行有研究报道[7]该种造模方法所造成的脾虚证模型症状与临床所见的脾虚症表现非常相似本实验亦利用此法用含有大黄药味的小承气汤耗伤大鼠脾胃元气以致脾虚究方面开展的工作还很少而临床研究却发现苍术具研究[8]表明脾虚证与胃肠炎症胃肠道的神经有很好的调理胃肠功能促进胃肠动力防治胃肠慢肌肉调控系统胃肠激素分泌障碍等多种病理生理改性炎症及溃疡性胃肠疾病的作用 [4] 目前临床应用变都有关系其主要临床表现为胃排空延迟和小肠传得较多的是麸炒苍术一般认为苍术麸炒后燥性会降输功能障碍本研究选取胃排空率及小肠推进比为指低而健脾作用则会增强但尚缺乏对其确切机制的标观察苍术对脾虚大鼠胃肠功能的影响实验中观进一步研究有文献[4]报道麸炒法可改变包括挥发察到脾虚模型大鼠胃排空及小肠推进率降低明显油在内的苍术的多种化学成分本研究从胃肠动力学 MTL 是一种很重要的胃肠激素具有促进胃肠运动方面对苍术麸炒前后防治脾虚证的作用进行比较以增强胃肠道收缩力和张力的作用 SP 是一种神经元阐明麸炒法是否可增强苍术的健脾作用及其可能的作递质具有兴奋胃肠运动的功能在整个胃肠道和肠用机制神经系统都有广泛的分布对几乎所有的消化道平滑脾虚证以运动消化吸收功能障碍作为主要表肌都具有刺激其收缩的作用 [9] SS 可调节胃肠蠕动现是一个多器官多系统功能障碍的综合性症候群通过抑制胃肠道激素和乙酰胆碱而起到间接调整胃肠其中消化系统障碍是这一症候群的中心有研究 [5] 道运动的作用[10] 三者均能很好的体现胃肠功能的变显示绝大部分的消化系统疾病以及能量代谢或免疫化实验表明脾虚可使上述胃肠激素表达明显异

6 ICC c- kit [16] ICC c- kit - c- kit [11] ATP ICC ATP [12] ATP ATP ATP ATP Ca 2+ CaM [13] Ca 2+ [14] Ca 2+ - CaM- MLCK MLCK MLCK MLCK Ca 2+ - CaM- MLCK Caja ICC ICC [15] ICC ICC C- kit ICC ICC

21 13 D / 2 10 ig ig 10 d 1. 0 g kg /2 2 β- 5 g kg g kg mg kg - 1 ig ig 24 h 5% 1 2% CMC-Na 2. 5 ml 30

21 13 D / 2 10 ig ig 10 d 1. 0 g kg /2 2 β- 5 g kg g kg mg kg - 1 ig ig 24 h 5% 1 2% CMC-Na 2. 5 ml 30 21 13 1 2 1* 2 1. 430065 2. 445000 20 10 5 g kg - 1 5 mg kg - 1 ig 10 d ig HE 1 TFF1 TFF1 P < 0. 01 P < 0. 01 P < 0. 01 P < 0. 05 R285. 5 doi 10. 13422 /j. cnki. syfjx. 2015130100 A 1005-9903 2015 13-0100-05