1410 HEREDITAS (Beijing) ancestry location of oversea Chinese, the surname origin and evolution, origin and migration of modern humans and man

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1 HEREDITAS (Beijing) , 34(11): ISSN 研究报告 DOI: /SP.J 人类 Y 染色体 36 个新 STR 位点的筛选与鉴定 1, 彭冬铂 2, 姜正文 2 1,, 孙斯平 2, 李才华 2, 卢大儒 1 1.,,, ; 2., 通过对 NCBI 数据库中的 Y-STR 位点和生物信息学预测的新 Y-STR 位点的分析, 选取了 133 个位点在 48 个全球分布的样本中进行检测验证, 获得 41 个可靠的高频位点, 其中 36 个为首次发现验证的新 Y-STR 位点利用这 41 个 Y-STR 位点, 在 200 个上海随机男性样本中共发现 200 种单倍型, 实现了人群内个体间 100% 区分, 并通过对浙江江山一姜氏聚集地的 9 个 5 代以内无血缘关系的姜氏个体和 7 个上海随机人群的姜氏个体的单倍型的分析, 发现 6 个江山姜氏个体具较近的亲缘关系 ( 彼此差异位点在 2~4 个之间 ) 这 41 个 Y-STR 位点具备较高的信息度, 能有效区分群体内不同家族来源的个体, 这将有助于群体内个体间近代亲缘关系研究, 并有望在法医学法医个体识别亲权鉴定, 人类起源迁徙等研究中发挥重大作用 Y-STR; 多态性 ; 单倍型 ; 区分度 ; 近代亲缘关系 Screening and identification of 36 new STR loci in human Y chromosome PENG Dong-Bo 1,2, JIANG Zheng-Wen 2, SUN Si-Ping 1,2, LI Cai-Hua 2, LU Da-Ru 1 1. State Key Laboratory of Genetic Engineering, MOE Key Laboratory of Contemporary Anthropology, School of Life Science, Fudan University, Shanghai , China; 2. Center for Genetic & Genomic Analysi, Genesky Biotechnologies Inc., Shanghai , China Abstract: 133 candidate Y-STR loci were selected from NCBI STS database or by bioinformatics analysis in human Y-chromosome sequence, and were screened among 48 DNA samples around the world. Forty-one Y-STRs with high allelic frequency were validated, 36 of which were first reported. Two hundred haplotypes of the 41 STRs were identified among 200 randomly sampled male individuals in Shanghai, indicating 100% inter-individual discrimination. By network analysis of haplotypes of the 41 STRs among nine Jiang-surname male individuals with no consanguinity within 5 generations from a Jiang-surname individual gathering at Jiangshan, Zhejiang Province, and 7 Jiang-surname male individuals from the random shanghai population, 6 Jiang-surname individuals from Jiangshan were close with only 2-4 STR locus difference. These 41 Y-STR loci provide enough information by which individuals from each other with different early modern family origin can be effectively distinguished. This will promote studies on identification of non-lineal relationship in forensics, 收稿日期 : ; 修回日期 : 作者简介 :,, Tel: ; pengdongbo@163.com 通讯作者 :,,,, drlu@fudan.edu.cn 致谢 : 网络出版时间 : :51:15 URL:

2 1410 HEREDITAS (Beijing) ancestry location of oversea Chinese, the surname origin and evolution, origin and migration of modern humans and many other studies of Contemporary Anthropology Keywords: Y-STR; polymorphism; haplotype; discrimination; early modern genetic relationship 1921, Painter [1] Y, Y bp, 95% (Nonrecombining portion of the Y chromosome, NRY) [2] Y,,, (Short tandem repeat, STR), DNA, 2~6,, STR 10-3 / [3, 4], SNP (SNP 10-9 / [5] ).,, [6~14] SNP,, STR, Y-STR,, Y-STR, DYS19 DYS388 DYS389I DYS389Ⅱ DYS390 DYS391 DYS392 DYS393 DYS425 DYS426 DYS434 DYS435 DYS436 DYS437 DYS438 DYS439 [15], Y-STR, NCBI DYS 3, Y-STR, DYS, 48, 200,,, 9 5 7, ( ) ,, 9, Y-STR 位点预测 (1) Y-STR, 201 DYS STR ; (2) 10 Y-STR ; (3) Y-STR NCBI 3 (Refercese HuRef Celera), Y-STR ; (4), Y-STR, 133 Y-STR, 30 DYS, 103, Y-STR 位点检测 PCR Y-STR, 5 4, 4 (FAM VIC NED PET) Primer3 (

3 11 : Y 36 STR 1411 primer3), PolyN SNP, 2 Y-STR (1) 20 μl PCR, 1 Hotstar Taq, 0.1 μmol/l PCR, 2.4 mmol/l MgCl 2, 0.3 mmol/l dntp, 1 U Hotstar Taq (QIAGEN), 5~10 ng DNA, ddh 2 O PCR min; s; s, 72 2 min, 10, 1 ; s, s, 72 2 min, 20 ; 72 2 h (2) 20 µl 1 HotStar Taq, 2.0 mmol/l MgCl 2, 0.2 mmol/l dntp, 0.1 µmol/l 4, 1 U Hotstar Taq (QIAGEN), 1 µl PCR, ddh 2 O min; s, s, 72 3 min, 30 (3) 10, LIZ500 (0.2 µl LIZ µl Hi-Di+1 µl PCR )95 5 min, ABI3130 l, GeneMapper4.0(Applied Biosystems ) Y-STR 数据分析 Y-STR 等位基因的命名 Y-STR DNA (ISFG DNA Commission) [16, 17] Y-STR PCR 1, 男性个体区分度 Y-STR PDM (Power of Discrimination for Males), Y-STR PDM, PDM 1 n =, X STR, PDM=1- [18] Network 软件分析样本之间遗传亲缘关系 Network, i X 2 i, ( ),, [19] Network MJ(Median-Joining network) RM(Reduced Median network) MJ Y-STR [20],,,,,, Y-STR 48,, 133 Y-STR 41 Y-STR ( 5 DYS, 36 ), 3, 1, 3 panel Y-STR PDM(Power of discrimination for males) 200, 41 Y-STR, ( 3), 100%, 41 Y-STR 36 Y-STR, 41 Y-STR (S85), ( 2) 3.3 Network 200, Network ( 3, ) 41 Y-STR,,,

4 1412 HEREDITAS (Beijing) 表 1 41 个 Y-STR 位点检测信息 STR# Repeat Unit Ref 1 HuRef 1 Celera (bp) S95 3 ac FAM 1 220~250 S115 gt FAM 1 286~326 S10 aata FAM 1 408~470 S96 ag VIC 1 270~312 S63 ac VIC 1 362~403 S31 ac VIC 1 430~470 DYS602 agat NED 1 228~268 S73 tg NED 1 278~312 S16 agat NED 1 372~424 S85 ctt PED 1 224~290 S6 tacta PED 1 280~336 S20 gata PED 1 390~420 S120 tg PED 1 470~490 S110 tg FAM 2 180~228 DYS390 agat FAM 2 264~308 S32 tg FAM 2 300~360 S4 aag FAM 2 398~440 S70 ctt VIC 2 178~232 DYS391 tcta VIC 2 270~310 S14 tatc VIC 2 372~428 DYS389 gata VIC 2 450~520 S91 gt NED 2 294~332 S5 tccctt NED 2 470~508 S99 ca PED 2 220~264 S64 gt PED 2 270~288 DYS448a19 agagat PED 2 300~390 S13 atag PED 2 390~432 S23 aat PED 2 460~490 S109 ac FAM 3 184~228 S76 ga FAM 3 220~260 S11 aaag FAM 3 310~360 S75 ca FAM 3 388~428 S92 gt FAM 3 420~446 S74 ac VIC 3 260~298 S81 gt VIC 3 370~394 S62 tttc VIC 3 412~476 S105 ac NED 3 208~272 S112 ag NED 3 360~390 S117 ac NED 3 460~484 S80 tg PED 3 224~298 S56 tttc PED 3 420~ (Ref HuRef Celera ) ; 2 NCBI GRCh37 Y ; 3 S 36

5 11 : Y 36 STR 1413 图 1 41 个 Y-STR 位点毛细管电泳分型检测峰图 A 1 13 Y-STR ; B 2 15 Y-STR ; C 3 13 Y-STR

6 1414 HEREDITAS (Beijing) 图 2 41 个 Y-STR 位点的男性个体区分度 (PDM) 图个随机上海男性样本的 Network 分析图 ( ) 41 Y-STR, Network 16 (16 4), 9 (P003 P008 P019 P037 P044 P047 P053 P058 P069), 5, 7 ( ) ( 4, 5) 6 (P008 P019 P037 P053 P044 P069), 2~4, P003 P047, 6, P058, 135,, Y-STR 3 Y DNA,

7 11 : Y 36 STR 1415 图 4 姜氏家系样本的遗传亲缘关系图图 5 姜氏家系亲缘关系密切样本之间的亲缘关系图 Y-STR, 3 NCBI DYS, 48, 200, 100% 41 Y-STR, 36 Y-STR 36 Y-STR 5 DYS,, 200,,,,, Y-STR Y, STR, Y,, SNP Y STR PCR, PCR,, 5,, PCR,,,, 36 Y-STR, 5 DYS 3 PCR, 200 9, 41 STR,, 1~4 ( 参考文献 (References): [1] Painter TS. The Y-Chromosome in mammals. Science, 1921, 27: DOI [2] Skaletsky H, Kuroda-Kawaguchi T, Minx PJ, Cordum HS,

8 1416 HEREDITAS (Beijing) Hillier L, Brown LG, Repping S, Pyntikova T, Ali J, Bieri T, Chinwalla A, Delehaunty A, Delehaunty K, Du H, Fewell G, Fulton L, Fulton R, Graves T, Hou SF, Latrielle P, Leonard S, Mardis E, Maupin R, McPherson J, Miner T, Nash W, Nguyen C, Ozersky P, Pepin K, Rock S, Rohlfing T, Scott K, Schultz B, Strong C, Tin-Wollam A, Yang SP, Waterston RH, Wilson RK, Rozen S, Page DC. The malespecific region of the human Y chromosome is a mosaic of discrete sequence classes. Nature, 2003, 423(6942): DOI [3] Garrigan D, Hammer MF. Reconstructing human origins in the genomic era. Nat Rev Genet, 2006, 7(9): [4] Cavalli-Sforza LL. The human genome diversity project: past, present and future. Nat Rev Genet, 2005, 6(4): DOI [5] Kayser M, Kittler R, Erler A, Hedman M, Lee AC, Mohyuddin A, Mehdi SQ, Rosser Z, Stoneking M, Jobling MA, Sajantila A, Tyler Smith C. A comprehensive survey of human Y-chromosomal microsatellites. Am J Hum Genet, 2004, 74(6): DOI [6],,,. 22 Y-STR., 2008, 30 (9): DOI [7],,,. 11 Y-STR., 2008, 30(5): DOI [8],,, Y-STR., 2009, 31(9): DOI [9],,,,. 7 Y-STR., 2007, 29(10): DOI [10],,,,,,. Y STR., 2006, 28(12): DOI [11],,,,. 2 Y-STR., 2006, 28(11): DOI [12] Grsković B, Mrsić G. Y chromosome: from evolution to forensics- an overview. Acta Med Croatica, 2010, 64(1): DOI [13] McElreavey K, Quintana-Murci L. A population genetics perspective of the Indus valley through uniparentallyinherited markers. Ann Hum Biol, 2005, 32(2): DOI [14] Gemmell NJ, Sin FY. Mitochondrial mutations may drive Y chromosome evolution. Bioessays, 2002, 24(3): DOI [15] Kayser M, Roewer L, Hedman M, Henke L, Henke J, Brauer S, Krüger C, Krawczak M, Nagy M, Dobosz T, Szibor R, de Knijff P, Stoneking M, Sajantila A. Charaeteristics and frequency of germline mutations at microsatellite loci from the human Y chromosome, as revealed by direct observation in father/son Pairs. Am J Hum Genet, 2000, 66(5): DOI [16],,. 17 Y-STR., 2011, 33(3): DOI [17] Bär W, Brinkmann B, Budowle B, Carracedo A, Gill P, Lincoln P, Mayr W, Olaisen B, DNA recommendations. Further report of the DNA Commission of the ISFG regarding the use of short tandem repeat systems. Forensic Sci Int, 1997, 87(3): DOI [18] Pericić M, Barać Lauc L, Martinović Klarić I, Janićijević B, Rudan P. Review of Croatian genetic heritage as revealed by mitochondrial DNA and Y chromosomal lineages. Croat Med J, 2005, 46(4): DOI [19] Scheinfeldt L, Friedlaender F, Friedlaender J, Latham K, Koki G, Karafet T, Hammer M, Lorenz J. Unexpected NRY chromosome variation in Northern Island melanesia. Mol Biol Evol, 2006, 23(8): [20] Bandelt HJ, Forster P, Röhl A. Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol, 1999, 16(1): DOI

9 11 : Y 36 STR 1417 附表个验证 STR 位点信息 STR# Repeat Unit Ref 1 HuRef 1 Celera 1 5 POS. 2 3 POS. 2 DYS19 tatc DYS197 tg DYS199 ca DYS209 tg DYS211 caa DYS257 tg DYS274 gt DYS280 tg DYS288 ac DYS289 ac DYS379 tg DYS385 gaaa DYS389 gata DYS390 agat DYS391 tcta DYS392 aat DYS393 tctct DYS395 agat DYS413 ac DYS426 tgt DYS437 tcta DYS438 ttttc DYS446a13 tc DYS447a22 ttata DYS448 agagat DYS448a19 agagat DYS458 tatc DYS463a18 ggaag DYS602 agat DYS80 agat S1 gaaag S10 aata S100 at S101 ta S105 ac S106 at S107 at S108 tg S109 ac S11 aaag S110 tg S111 tc S112 ag S115 gt

10 1418 HEREDITAS (Beijing) STR# Repeat Unit Ref 1 HuRef 1 Celera 1 5 POS. 2 3 POS. 2 S116 tg S117 ac S118 ac S119 tg S12 tatc S120 tg S124 gt S125 tg S127 at S128 ta S13 atag S14 tatc S15 aaat S16 agat S17 ttta S18 atcc S19 tgtt S2 ac S20 gata S21 tcta S22 tct S23 aat S24 gga S25 ta S26 at S27 ta S28 at S29 ta S3 ta S30 ta S31 ac S32 tg S33 ta S34 at S35 at S36 ta S37 ac S38 ta S39 ta S4 aag S40 tg S41 ct S42 ac S43 gt S44 tg

11 11 : Y 36 STR STR# Repeat Unit Ref 1 HuRef 1 Celera 1 5 POS. 2 3 POS. 2 S46 ta S48 ta S49 at S5 tccctt S52 tg S53 gt S54 ac S55 at S56 tttc S57 tg S6 tacta S60 ta S61 ct S62 tttc S63 ac S64 gt S67 ac S68 ac S70 ctt S73 tg S74 ac S75 ca S76 ga S77 tg S79 ta S8 cttt S80 tg S81 gt S82 gt S83 at S85 ctt S86 at S89 ca S9 tttc S90 tg S91 gt S92 gt S93 ta S94 ac S95 ac S96 ag S97 ta S98 ac S99 ca (Ref, HuRef, Celera) ; 2 NCBI GRCh37 Y

12 1420 HEREDITAS (Beijing) 附表 2 41 个 Y-STR 引物信息 (5-3 ) 1 S95F CTTTCGGCCCAGTAAGGGTATTCAAGGCTGCTAGTCATGCTTCTA 1 S95R GTTTGCTTTGAAGCGTGTTTGAAAGAA 1 S115F CTTTCGGCCCAGTAAGGGTATGGTCAACAGAAGGGCTTGTTATTTTC 1 S115R GTTTAGCCCCCTCAATTAGCCTTTTG 1 S10F CTTTCGGCCCAGTAAGGGTATCCTCCCCACATTCTGAAATCAC 1 S10R GTTTCCAAGAGATTCAGAGGCCTCCAC 1 S96F TAGACTAAACGGGCGTGGTTCTGCTGGTGCCTGATGACAGA 1 S96R GTTTGGCCCTGGGGAAAATAAGATCA 1 S63F TAGACTAAACGGGCGTGGTTCGCAGGAGAGCAATGCCTTGTAA 1 S63R GTTTtgatggagatgggttgctgaag 1 DYS602F ATGAGACTACGCGGCTAATGCTGAGTGGGAGAAATGGATGACA 1 DYS602R GTTTTTTTTGGGCCCTGCATTTTG 1 S31F TAGACTAAACGGGCGTGGTTCTGTCTGATGATGCCCAAAATGA 1 S31R GTTTGGAAAAACAGACCCAGTCACAAA 1 S73F ATGAGACTACGCGGCTAATGCCTCAAGAGCTGCCAGGCTGTGT 1 S73R GTTTGCCTGCCTCCCAATTGACTAAA 1 S16F ATGAGACTACGCGGCTAATGCACAGTGTATCCGCCATGGTAGC 1 S16R GTTTGGGATGGGAAATGATGTTTCTGA 1 S85F GTGTTCCGACCGCTATTGTTTGACTCCCCCATGCCAGTAAGAA 1 S85R GTTTGGGAACTGAGGGAAGGCAAAA 1 S6F GTGTTCCGACCGCTATTGTTTGGGGCCTTACTTACATTGGTGGT 1 S6R GTTTTGGGTGCTAAATGCCCAAGAGT 1 S20F GTGTTCCGACCGCTATTGTTTTCCACAATGCTTTCCTTACACCTG 1 S20R GTTTAACCAGTGAGAGCCGGAACCTC 1 S120F GTGTTCCGACCGCTATTGTTTCAAACAGGCAGGCTCCTCCTAA 1 S120R GTTTaggATTTCCCTCACCATTTTCTG 2 S110F CTTTCGGCCCAGTAAGGGTATCCCCGGGCTTATTTCAGATTTA 2 S110R GTTTTGTGAGCCAGCCACACTTGATA 2 DYS390F CTTTCGGCCCAGTAAGGGTATACAGTGTATCCGCCATGGTAGC 2 DYS390R GTTTCATTTTTGGGCCCTGCATTT 2 S32F CTTTCGGCCCAGTAAGGGTATCAGCAGGGAGGACTTGGACAAA 2 S32R GTTTgctttcttccaaccatccattca 2 S4F CTTTCGGCCCAGTAAGGGTATTTTGAAAGCTGGTGGGATTCAG 2 S4R GTTTAGCAGCTGCCAGGTTGTGAA 2 S70F TAGACTAAACGGGCGTGGTTCAGGAATGTGGCTAACGCTGTTCA 2 S70R GTTTAATCAGAACACAGAGCCCCACAA 2 DYS391F TAGACTAAACGGGCGTGGTTCccccaccacagattagcattca 2 DYS391R GTTTagggataggtaggcaggcagat 2 S14F TAGACTAAACGGGCGTGGTTCTCAGGAATTTGCTGGTCAATCTC 2 S14R GTTTGGAAAATTTTGACAAGCCCAAAG 2 DYS389F TAGACTAAACGGGCGTGGTTCGAATGTCatagatagatgatggactgc 2 DYS389R GTTTttgCATTAGCATGAGAGATCctg

13 11 : Y 36 STR (5-3 ) 2 S91F ATGAGACTACGCGGCTAATGCCCTCACAGGAAGCAGAATTCACTACA 2 S91R GTTTTCTTTGTTTCAAGTTGGCCaattt 2 S5F ATGAGACTACGCGGCTAATGCTCCCTAAAAGTTACAACCCAGCAC 2 S5R GTTTCACAGCAGTggctaggcacag 2 S99F GTGTTCCGACCGCTATTGTTTATGGTCCCTCTTAATGTTGCCTGT 2 S99R GTTTTCTTTTGTCTGGGGTATGACAGCTA 2 S64F GTGTTCCGACCGCTATTGTTTAATGCATTAACAGCCCATCCCATTAG 2 S64R GTTTgaaggaaccagcacttcccata 2 DYS448a19F GTTTAGAAATAGAGATCGCgagacagaaag 2 DYS448a19R GTGTTCCGACCGCTATTGTTTTGATTCCCTGTGTTGGAGACCTTTTC 2 S13F GTTTcctcccttacCACAGATAGGTAAGACA 2 S13R GTGTTCCGACCGCTATTGTTTTGAAGATGTTCAGTCAGGAAAAAAAA 2 S23F GTGTTCCGACCGCTATTGTTTCGCATGGACTTCCACCCACTTA 2 S23R GTTTTTCCAAGCCGTTAATTTCCTCAT 3 S109F CTTTCGGCCCAGTAAGGGTATAAGCTGTTACCACCTGCTTACATAGAA 3 S109R GTTTGAGATGAAGCTTCTTTGAGGCATAA 3 S76F GTTTGGGTTCACTCTAGGTGGACAGAAAA 3 S76R CTTTCGGCCCAGTAAGGGTATTCACAGGGGTCCAAAGGAAAC 3 S11F GTTTcctacctgggcaacagaacaaca 3 S11R CTTTCGGCCCAGTAAGGGTATCCCAGACAGAATGCAATCTTCttt 3 S75F GTTTCATCATTCTATATGAAGCAGTGCAAAA 3 S75R CTTTCGGCCCAGTAAGGGTATGTGATAAAGGCTTGGCAAAAAGAGA 3 S92F CTTTCGGCCCAGTAAGGGTATTTTGCACCTCTGGTCTTACTGGTC 3 S92R GTTTACCTGCCATCGAAAGCATTACC 3 S74F TAGACTAAACGGGCGTGGTTCGATCCCACAGCCCAAGCAGAT 3 S74R GTTTGGGACTTCTGCAAAACCATACCA 3 S62F TAGACTAAACGGGCGTGGTTCGCCTGGGACCGTGTCTTTATTT 3 S62R GTTTGGTGGCAACCTAAGCTGAAATG 3 S81F TAGACTAAACGGGCGTGGTTCGGGCCTGGCAATCTTTTCAC 3 S81R GTTTTGACCCTTTCCACAGACCCTTAAC 3 S105F ATGAGACTACGCGGCTAATGCGGCCATTCAGTTCTTCATCAGCTC 3 S105R GTTTTGATGAGAAACCACCACTGTAACTAGG 3 S112F ATGAGACTACGCGGCTAATGCTGACCAAAGCAAATCCTGAAATATG 3 S112R GTTTTCCCCAAAGGCAACCAAGAA 3 S117F ATGAGACTACGCGGCTAATGCTCTGCCAACCCACCTGATGATA 3 S117R GTTTTGACtgatttgctcacaatcacatatc 3 S80F GTGTTCCGACCGCTATTGTTTAGGCCCAATTTTTTGCACACAG 3 S80R GTTTGGCTGAAGTGCATTAGCCACATT 3 S56F GTGTTCCGACCGCTATTGTTTCATGTGCAAGGCAAAAGTACCTC 3 S56R GTTTCAGTTGTGGTACAGGCTGGATTT

14 1422 HEREDITAS (Beijing) 附表个上海随机男性样本单倍型信息 S5 S70 S73 S74 S75 S76 S14 S80 S81 S40 DYS391 S85 DYS389 S91 S92 S95 S96 S

15 11 : Y 36 STR S5 S70 S73 S74 S75 S76 S14 S80 S81 S40 DYS391 S85 DYS389 S91 S92 S95 S96 S

16 1424 HEREDITAS (Beijing) S5 S70 S73 S74 S75 S76 S14 S80 S81 S40 DYS391 S85 DYS389 S91 S92 S95 S96 S

17 11 : Y 36 STR S5 S70 S73 S74 S75 S76 S14 S80 S81 S40 DYS391 S85 DYS389 S91 S92 S95 S96 S

18 1426 HEREDITAS (Beijing) S5 S70 S73 S74 S75 S76 S14 S80 S81 S40 DYS391 S85 DYS389 S91 S92 S95 S96 S

19 11 : Y 36 STR S99 S13 S32 S105 S11 S109 S110 DYS602 DYS390 S112 S23 S115 S117 S6 S10 S120 S4 DYS448a

20 1428 HEREDITAS (Beijing) S99 S13 S32 S105 S11 S109 S110 DYS602 DYS390 S112 S23 S115 S117 S6 S10 S120 S4 DYS448a

21 11 : Y 36 STR S99 S13 S32 S105 S11 S109 S110 DYS602 DYS390 S112 S23 S115 S117 S6 S10 S120 S4 DYS448a

22 1430 HEREDITAS (Beijing) S99 S13 S32 S105 S11 S109 S110 DYS602 DYS390 S112 S23 S115 S117 S6 S10 S120 S4 DYS448a

23 11 : Y 36 STR S99 S13 S32 S105 S11 S109 S110 DYS602 DYS390 S112 S23 S115 S117 S6 S10 S120 S4 DYS448a

24 1432 HEREDITAS (Beijing) S99 S13 S32 S105 S11 S109 S110 DYS602 DYS390 S112 S23 S115 S117 S6 S10 S120 S4 DYS448a 附表 4 16 个姜氏样本 41 个 Y-STR 单倍型信息 S1 S2 S3 S4 S5 S6 S7 S8 S9 S10 S11 S12 S13 S14 S15 S16 S17 S18 S19 S20 S P P P P P P P P P S22 S23 S24 S25 S26 S27 S28 S29 S30 S31 S32 S33 S34 S35 S36 S37 S38 S39 S40 S41 S P P P P P P P P P

Microsoft Word - 03zc0403.doc

Microsoft Word - 03zc0403.doc ( ) 33 4 SIENE IN HINA ( Series ) 2003 8 Y DNA * ** ** (- 650091; 650091; 200433; 650031; enter for Genome Information Department of Environmental Health University of incinnati incinnati OH 45267 USA;