Microsoft Word 杨芳-二校出4173

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1 6 10 Vol. 6 No Journal of Food Safety and Quality Oct., 2015 杨芳 1, 严世武 2, 马维思 2, 杨斌 2* (1., ; 2., ) 摘要 : 目的 10,,, 方法, 结果 17 1 γ-, 7, 9.25%~19.74%, 3.97%~8.63%,,,, FAO/WHO (SRC) 78.22, +, 62.74%~66.48% (E/T) (E/N) SRC, 4 结论,, 关键词 : ; ; ; Amino acid composition and nutritional evaluation of Laggera pterodonta (DC.) Benth. from different areas in Yunnan YANG Fang 1, YAN Shi-Wu 2, MA Wei-Si 2, YANG Bin 2* (1. Agri-Food Quality Standard & Testing Technology Institute, Yunnan Academy of Agricultural Science, Kunming , China; 2. Institute of Medicinal Plants Research, Yunnan Academy of Agricultural Sciences, Kunming , China) ABSTRACT: Objective To detect and evaluate the nutrition quality of Laggera pterodonta (DC.) Benth from 10 regions of Yunnan through amino acids composition and content for providing a theoretical basis of the utilization and quality control of Laggera pterodonta (DC.) Benth. Methods Amino acid composition was analyzed with automatic amino acid analyzer. Ratio coefficient of amino acid method and cluster analysis were conducted to evaluate nutrition quality and distinguish differences among the heterotrophic strains of L. pterodonta (DC.) Benth.. Results All samples had 17 kinds of amino acids and γ-aminobutyric, including 7 kinds of essential amino acids. The total amino acids content in L. pterodonta (DC.) Benth. from different regions was in range from 9.25%~19.74%, the essential amino acids content was in range from 3.97% to 8.63%. 基金项目 : (2010AE003) Fund: Supported by the Science and Technology Innovation Project of Yunnan Province (2010AE003) and the Yunnan Province Technological Innovation Talent Training Project * 通讯作者 :,, yb0872@163.com *Corresponding author: YANG Bin, Associate Research Fellow, Cultivation and Quality control of Medicinal Plants, Institute of Medicinal Plants Research, Yunnan Academy of Agricultural Sciences, No. 2238, Beijing road, Kunming , China. yb0872@163.com

2 Although there were significant differences between total amino acids and essential amino acids from different region, the L. Pterodonta (DC.) Benth contained almost all essential amino acids in a well-balanced proportion, and the essential amino acid composition exceeded the FAO/WHO recommended pattern. With amino acids ratio coefficient, the score of amino acids ratio coefficient SRC was 78.22, the first limiting amino acids were Met+Cys, the medicinal amino acids took up 62.74%~66.48% of the total amino acids. In addition, the multivariate statistical method was used to analyze the differences in amino acid content, essential amino acid content, medicinal amino acid content, the ratio of essential amino acids and total amino acids (E/T), the ratio of essential amino acids and non essential amino acids (E/N)and SRC among the samples from 10 regions. These geographic origins were classified into 4 categories by cluster analysis. Conclusion L. pterodonta (DC.) Benth. has a high nutritional value and health benefit, and can be processed into natural green products. KEY WORDS: Laggerapterodonta (DC.) Benth; amino acids; content analysis; nutritional evaluation 1 引言 (Laggera pterodonta (DC.) Benth.), [1],,,, [2] [3] [4] [5],, [6], 2 材料与方法 2.1 实验材料与试剂 : 10, (Laggera pterodonta (DC.) Benth.) 10~15,, 60, 60,, 1 : (2.5 µmol/ml 1.25 µmol/ml, Sigma ); γ- ( 99%, Sigma ); (, Sigma ); (, ); (, ) Table 1 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 表 1 臭灵丹草样品 The samples of Laggera pterodonta (DC.) Benth. 2.2 仪器与设备 L-8800 ( ); BSA224S-CW ( ( ) ); HG101-2 ( ) 2.3 实验方法标准溶液的配制 2.5 µmol/ml 1 ml 2.5 µmol/ml γ- 1 ml,, 0.02 mol/l 25 ml, 0.1 mmol/l 0.45 μm, 20 μl

3 10, : 样品溶液制备,, 100 mg 20 ml, 10 ml 6.0 mol/l,,,, (110±2) 22 h, 6.0 mol/l ph, 25 ml 1 ml 1 ml 0.02 mol/l, 0.45 μm, 20 μl L 色谱条件 (4.6 mm 60.0 mm), : 57.0, : 结果与分析 3.1 不同产地臭灵丹草中氨基酸组成及含量分析 1,, 18, 2 10, 17 (, ) 1 γ-, (T) 9.25%~19.74%, 14.29%, [6] [7] [8] [9] [10], C4, 19.74%, C1, 9.25% 10,, ; 2,, 7, 3.97%~8.63%, C4, C1, 22.25%,, 2.79% E/T 42.15%, E/N 72.86%, FAO/WHO, E/T 40%, E/N 60%,

4 : a ; b ; nm 440 nm 1 Fig. 1 Amino acid analysis spectrogram of standard and Laggera pterodonta (DC.) Benth. Table 2 表 2 不同产地臭灵丹草中氨基酸组成和含量 Composition and content of amino acid in Laggera pterodonta from different areas /% C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 Asp# Thr* Ser Glu# Gly# Ala Cys Val* Met*# Ile*# Leu*#

5 10, : 4177 续表 2 /% C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 Tyr Phe*# Lys*# His Arg# Pro T E N M E/T(%) E/N(%) M/T(%) γ- GABA# : 1.* ; 2.# ; 3.E/T ; 4.E/N ; 5.M/T 3.2 臭灵丹草中氨基酸的营养评价不同产地臭灵丹草中各种必需氨基酸与 FAO/WHO 推荐的氨基酸模式谱的比较,, [9], FAO/WHO [11] 3, C3 ;, +,,, C 氨基酸比值系数法对不同产地臭灵丹草中必需氨基酸营养价值的评价, [13] FAO/WHO, (RAA) (RC)(SRC) 10 RAA RC SRC 4 4, SRC 78.22, SRC [12], C1, 83.62, C3, C10, 4, + RC,, + +,,,, 3.3 不同产地臭灵丹草中药效氨基酸含量 2,, 9.24%, [7] [9] [10] [13] 62.74%~66.48%, 3

6 Table 3 表 3 各种必需氨基酸与 FAO/WHO 推荐氨基酸模式谱比较 Comparison of essential amino acid composition in Laggera pterodonta and FAO/WHO recommended pattern Thr Val Met+Cys Ile Leu Phe+Tyr Lys FAO/WHO C C C C C C C C C C Table 4 表 4 各种必需氨基酸的 RAA RC 及 SRC RAA, RC and SRC of essential amino acids in Laggera pterodonta Thr Val Met+Cys Ile Leu Phe+Tyr Lys SRC C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 RAA RC RAA RC RAA RC RAA RC RAA RC RAA RC RAA RC RAA RC RAA RC RAA RC RAA RC

7 10, : 4179,,,, [14] ;,,,,, [7] ;, [14] C4, 12.93%, 65.50%, C1, 5.91% 2, γ- (GABA), 0.07% GABA [15] C9 C10 GABA, 0.12%, C4, 0.03% GABA, 3.4 氨基酸聚类分析 SPSS 19.0, ward 10, ( 2) E/T E/N SRC, 5, 4 Ⅰ (C2 C9 C5),,, ; Ⅱ (C1 C3 C8),,, ; Ⅲ (C6 C7 C4),,, ; Ⅳ (C10),,, 1, Ⅰ, Ⅱ, Ⅲ, Ⅳ,,, : ;,,,, 2 Fig. 2 Dendrogram of Laggera pterodonta from 10 different geographic origins 4 结论 ( ), 17 1 γ-, ~19.74%, 3.97~8.63%,, +,,, SRC 78.22,,, 9.24%, 62.74%~66.48%, 3,,

8 4180 6,,, 参考文献 [1]. 75 [M ]. :, Chinese Academy of Science Commission. The flora of China (vol 75) [M]. Beijing: Science Press, [2].( )[S]. Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China (First Vol) [S]. [3],,,. [J]., 2008, 2(8): 14. Zhao J, Mao XJ, Yang TM, et al. Pharmacological action of Laggera pterodonta (DC.) Benth. [J]. Chin J Mod Drug Appl, 2008, 2(8): 14. [4],,, 3, 5- -6, 7, 3, 4 - CNE [J]., 2011, 38(3): Cao CS, Shen WZ, Li YL, et al. 3, 5-Hydroxy-6, 7, 3, 4 -tetramethoxyflavone isolated from Laggera pterodonta induces CNE cell apoptosis [J]. Progress Biochem Biophys, 2011, 38(3): [5],,,. [J]., 2013, 29(6): Li SH, Zhao Q, Liu F, et al. Structural identification and antioxidant activity of the flavones from Laggera pterodonta [J]. Mod Food Sci Technol, 2013, 29(6): [6],. [J]., 2013, 35(4): Tu XL, Fan QJ. Determination of amino acid contents in Gastrodia elata from different region [J]. Amino Acid Biot Resour, 2013, 35(4): [7],,,. [J]., 2014, 35(15): Yang X, Zhu HF, Wang T, et al. Comparative analysis of amino acid composition and nutritional value of roots of Codonopsis pilosula from Wushan and other growing regions in China [J]. Food Sci, 2014, 35(15): [8]. [J]., 2013, 52(16): Zhao T. Determination of amino acids in jujube from different habitats [J]. Hubei Agric Sci, 2013, 52(16): [9],,. [J]., 2011, 32(15): Chen R, Wu QN, Shen B. Amino acid composition and nutritional evaluation of Semen Euryales from different regions [J]. Food Sci, 2014, 35(15): [10],,,. [J]., 2014, 39(2): Wang YM, Wang Y, Ren XW, et al. Comparison of amino acid contents in different cultivars of Lycium barbarum L. fruit [J]. Food Sci Technol, 2014, 39(2): [11] Food and Agriculture Organization of the United Nations /World Health Organization. Energy and protein requirements [R]. Geneva: World Health Organization, 1973(WHO Technical Report Series, No. 522): 63. [12],. - [J]., 1988, 10(2): Zhu ST, Wu K. Nutritional evaluation of protein-ratio coefficient of amino acid [J]. Acta Nutrimenta Sin, 1988, 10(2): [13],,,. [J]., 2009, 31(1): Wang YH, Zhang ZH, Jin H, et al. Determination of amino acids in different parts of Physalisa ikekengi [J]. Amino Acid Biot Resour, 2009, 31(1): [14]. [J]., 1982(4): Wang JH. Medical amino acids [J]. World Clin Drug, 1982(4): [15],. γ- [J]., 2006, 40(3): Zhang H, Xu MY. The functions progress of γ-aminobutyric acid [J]. J Harbin Med Univ, 2006, 40(3): 作者简介 ( 责任编辑 : 李振飞 ) 杨芳, 助理研究员, 主要研究方向为农产品营养品质与质量控制 yfyb917@163.com 杨斌, 副研究员, 主要研究方向为药用植物栽培与质量控制 yb0872@163.com

Microsoft Word 杨旭昆_二校勿动版

Microsoft Word 杨旭昆_二校勿动版 7 10 Vol. 7 No. 10 2016 10 Journal of Food Safety and Quality Oct., 2016 7 杨旭昆 1, 汪禄祥 1*, 刘艳芳 1, 邵金良 1, 罗红 (1., 650205; 2., 650205) 2 摘要 : 目的 7 17 方法, 7, / (WHO/FAO) 7 结果 7 ; 6, ;, 6 20%,, 28.65%; 7, 5