)ng/μlvs.(0 21±0 04)ng/μl;(0 05±0 01)ng/μlvs. (0 08±0 01)ng/μl;(0 25±0 05)ng/μl vs. (0 33±0 05) ng/μl;alp<0 05).Conclusions Thelevelofintestin

Size: px

Start display at page:

Download ")ng/μlvs.(0 21±0 04)ng/μl;(0 05±0 01)ng/μlvs. (0 08±0 01)ng/μl;(0 25±0 05)ng/μl vs. (0 33±0 05) ng/μl;alp<0 05).Conclusions Thelevelofintestin"

喜品阮
7 years ago
Views:

1 236 *+,-./01-23& :; 程靖李枫林张宝管石侠侯丽丽蒋建华,-./ :1 - (NAFLD) &423 -<5678-9:=>,?@5678-9A=B NAFLDC DE SD&4 12 FGHIJK0LMN,-M,0LMO./,,-M,-,P 8QR ST&4,HEUV WM&4 X YA=, - :=>, ZV[J\I ]W M&4^_78-9`a:=>,-M&4 bc [(139 6±17 29) U/L (37 67±5 28)U/L] b [(367±49 36) U/L (97 67±13 92) U/L] [(0 83±0 08)mmol/L (0 60±0 04)mmol/L] [(6 34±0 53)mmol/L (1 77± 0 29)mmol/L] - [(0 31±0 03)mmol/L (0 22±0 01)mmol/L] 0LM,, ' (P<0 05); 0LMZ,,-M^_ 9 c9n 78-9 (SCFA) [(0 16±0 03)ng/μl (0 21±0 04)ng/μl; (0 05±0 01) ng/μl (0 08±0 01)ng/μl;(0 25±0 05) ng/μl (0 33±0 05) ng/μl], ' (P< 0 05),-./ : NAFLD23&456 SCFA`a,^_ SCFA: 0 NAFLD: :,-./;1 - ;78-9 :"# % ( ) Efectofhigh fatdietontheintestinalshort chainfatyacidsinnon alcoholicfatyliverdiseaserats ChengJing,LiFengling,ZhangBao,GuanShixia,HouLili,JiangJianhua.DepartmentofClinicalNutri tion,thefirstafiliatedhospitalofanhuimedicaluniversity,hefei230022,china Corespondingauthor:JiangJianhua,E mail:jjhua@yeah.net Abstract Objective Toobservethechangesofbloodlipidandintestinalshort chainfatyacidsin ratswithnon alcoholicfatyliverdisease(nafld)inducedbyhigh fatdiet,andtoexploretheroleofintes tinalshort chainfatyacidsinthepathogenesisofnafld.methods MaleSDrats(n=12)wererandom lydividedintocontrolgroupandhigh fatgroupaccordingtotherandom numbertable.thecontrolgroup wasfedwithnormaldiet,whilethehigh fatgroupwasfedwithhigh fatdiet.theratsweresacrificedatthe endofeighthweekafterexperiment.hestainingwasusedtoobservethepathologicalchangesoftheliver inthetwogroups,thechangesinserum lipidprofileweremeasured,andthechangesofthecontentsof fecesshort chainfatyacidsinthetwogroupsofratsweredeterminedbygaschromatography.results The levelsofserumalaninetransaminase[(139 6±17 29)U/Lvs.(37 67±5 28)U/L],aspartatetransami nase[(367±49 36)U/Lvs.(97 67±13 92)U/L],triglyceride[(0 83±0 08)mmol/Lvs.(0 60± 0 04)mmol/L],totalcholesterol[(6 34±0 53) mmol/lvs. (1 77±0 29) mmol/l],andvery low densitylipoprotein[(0 31±0 03)mmol/Lvs.(0 22±0 01)mmol/L]inthehigh fatgroupweresignifi cantlyhigherthanthoseinthecontrolgroup(p<0 05).Comparedwiththecontrolgroup,thecontentsoface ticacid,propionicacidandtotalshort chainfatyacidsinthehigh fatgroupweresignificantlylower[(0 16± DOI: /cma.j.isn X :230022!,"#$%&' $ % :(),E mail:jjhua@yeah.net

2 )ng/μlvs.(0 21±0 04)ng/μl;(0 05±0 01)ng/μlvs. (0 08±0 01)ng/μl;(0 25±0 05)ng/μl vs. (0 33±0 05) ng/μl;alp<0 05).Conclusions Thelevelofintestinalshort chainfatyacidsis reducedinthenafldratsinducedbyhigh fatdiet.monitoringoffecesshort chainfatyacidsmayplayan importantroleinthediagnosisandtreatmentofnafld. Keywords High fatdiet;non alcoholicfatyliver;short chainfatyacid Fundprogram:AnnualKeyScientificResearchProjectofAnhuiProvince( ) 1 - (non alcoholicfatyliver disease,nafld) K B X :H, Z [1],-./ 1 - :,,-. / O!, C1 - : 56 :A=B DE : V./:A=056 : &;, ] :./ C =>, >6 : C Z : = > [2] (short chainfatyacids,scfa) 56 C 5 > : > N SCFA 56 1: [3], 9 c9 9! " K,,- < #$%/ & 56' < ' :C 56 SCFA [4] 0^_ SCFA: 0 ' : : ( )* SD&4 12,4+ 7Q, a 160~200g,,-"#$%&' /0 /0K1 30% 36% 9% 2 20% 34 3% 5 2%N6 0 75kg/ 500kg, 7aK 13 55kJ/g,- /0K: 80% +20%8, - > 9 J:K ; ; 9 c9 9N 9 K Aldrich chemicalcompany<= ; >? GC 2010 ZV[> (@A B<=), C DE >? 1 2 0I JM<23) ( )* SD&4 12, 3, J B 23~26 : F,G 1QH I, FGHIJ 12 &4J 0LM N23M, M 6,0LM O, 23M,- NAFLD&423,- NO / 1, NP HQ, QR 1, 8Q ASY 8QR,WM&4S/ 12h, T U V-W XY: ^_PT, -70 &4 Z [ IST, \ IP -70 ]^ ;V _`Ma,10%bc ] < ]0I V XMa, ]& L, 'A=;V X,RaP F (P / a 100%);Vbc ]: Ma,,,HEUV, VZ [,JE, - >J\> ] > bc (alaninetransaminase,alt) b (glutamic oxaloacetic transaminase, AST) (triglyceride,tg) (totalcholesterol,tc) - (very low densitylipoprotein,vldl) Z (fasting bloodglucose,fbg) ; 0I [5], ^_ 0 5gP 10mlE., 2 5ml, J & 3min, ph 2~3, 10min, 1 5ml E.,5000r/minE. 20min;V 1ml P", F 9, F K 1mmol/L, ZV[J\ V[ : (30m 0 53mm,0 5μm) ] E, 100, 0 5min,WIO 8 /min 180, 1min, J ]0,WIO 20 /min: 200,

3 238 5min : J: 240 N 200 ; ak 5μl; :, K 3ml/min F SYB HPChemStation ZV[ 0 ], I ]a, F IJ: SCFA:`a 1 3 'SY SPSS11 0 J\,F x±sh, J =am t P<0 05K ' &4 an F=> 2 0LMN,-M&4 at, DEWM&4 a,4q!wm&4 a T,4QI,-M&4 a &P0LM&4, 8QRWM& 4 at (P>0 05) ( 1) 8QR,, -M&4 F 0LM&4,, ' (P<0 05)( 2) 2 2 X N Y'A= 0LM&4 X V,,, A=;,-M&4 X &,V X, V,H, < A=, =, ( 3) 0LM ` A ; 23M X Y T, A=, `,,, P F&- = ( 4) 2 3 > : 0LMZ,,-M&4 ALT AST TG TC VLDL,, ' (P<0 05);WM T ' (P>0 05)(H 1) 2 4 SCFA: 0LMZ,,-M&4^_ 9 c9n SCFA, ' (P<0 05);WM 9 T ' (P>0 05)(H 2 5) 3 "! G : 0 N./!" # N NAFLD:$ [6],-./ O! 1 &4 a:=> Fig1 Changesofbodymasinrats A 0LM;B,-M! 3 &4 X '=> Fig3 Morphologicalchangesofratlivers : 0LM, a P<0 05! 2 &4 F:=> Fig2 Changesofratsliverindex A 0LM;B,-M! 4 &4 X Y'A= (HEUV, 100) Fig4 Pathologicalchangesofratlivers(HEstaining, 100)

4 239 # 1 WM&4 > (x±s) Table1 Comparisonofbiochemicalindicatorsbetweenthetwogroups(x±s) M: ALT(U/L) AST(U/L) TG (mmol/l) TC (mmol/l) VLDL(mmol/L) FGB (mmol/l) 0LM (n=6) 37 67± ± ± ± ± ±0 42,-M (n=6) ±17 29 a ±49 36 a 0 83±0 08 a 6 34±0 53 a 0 31±0 03 a 6 33±0 22 :ALT:bc ;AST:b ;TG: ;TC: ;VLDL: - ;FGB: Z ; 0LM, a P<0 05 # 2 WM&4 SCFA (x±s) Table2 ComparisonofSCFAbetweenthetwogroups(x±s) M: 9 (ng/μl) c9 (ng/μl) 9 (ng/μl) SCFA (ng/μl) 0LM (n=6) 0 21± ± ± ±0 05,-M (n=6) 0 16±0 03 a 0 05±0 01 a 0 04± ±0 05 a :SCFA: 78-9; 0LM, a P<0 05 :SCFA: 78-9;A 0LM;B,-M! 5 WM&4 SCFA %& :=> Fig5 ComparisonofSCFAsignalintensitybetweenthetwogroups!, C NAFLD 56 B NAFLD:C C' : C,,- > :./ O 56 :A= [7] 56 : SCFA 5 4 X 5. : 7,7K / 10%(_:7a 56 )* +3: : SCFA*+NFa Z), D 56 SCFA:=>, O,5656 :=> A D,-./ ) NAFLD 2 3, D,-M&4 X &,- < A=, D HEUV O./023M&4 X- 1,- FN -,, D,-./ ) # NAFLD 23 D0WM&4^_ SCFA : C,23M&4 9 c9< : SCFA `a 0LM ' C,-. / Fa +, 9`a [8] 5F SCFA, c9n9, O ; 7a-4, 5 an -`a [9 10] 0! &4 Oc967I C, ]E :A9 [11] c9 : O;\ X- [12], : 5- M a: [13] 0 ( 56F SCFA K J\,/ K : - #$ SCFA: TZ, 56 SCFA: Z / DA =56 M : SCFA B

5 240 [3] ^_ SCFA 56 :; 56 : <=] SCFA:`aN* + NAFLD^_ SCFA :>,-./ A B.,-./ : NAFLD23&456 SCFA 7 # NAFLD:C, CK NAFLD: D # :E, O ; SCFA: N : 0 : D FNGH,0 SCFA: N I J, K0 DLMN NAFLD: : $ % & ' [1] BugianesiE,MoscatieloS,CiaravelaMF,etal.Insulinresistance innonalcoholicfatyliverdisease[j].curpharmdes,2010,16(17): DOI: / [2]WalkerAW,InceJ,DuncanSH,etal.Dominantanddiet re sponsivegroupsofbacteriawithinthehumancolonicmicrobiota [J].ISMEJ,2011,5(2): DOI: /ismej [3]McoristAL,AbelGC,CookeC,etal.Bacterialpopulationdy namicsandfaecalshort chainfatyacid(scfa)concentrationsin healthyhumans[j].brjnutr,2008,100(1): DOI: /S [4]TanJ,McKenzieC,PotamitisM,etal.Theroleofshort chain fatyacidsinhealthanddisease[j].advimmunol,2014,121: DOI: /B [5]ZhaoG,NymanM,J nsonja.rapiddeterminationofshort chainfatyacidsincoloniccontentsandfaecesofhumansandrats byacidifiedwater extractionanddirect injectiongaschromatogra phy[j].biomedchromatogr,2006,20(8): DOI: /bmc.580. [6]Henao MejiaJ,ElinavE,ThaisCA,etal.Theintestinalmicrobi otainchronicliverdisease[j].advimmunol,2013,117: DOI: /B [7]WuGD,ChenJ,HofmannC,etal.Linkinglong term dietary paternswithgutmicrobialenterotypes[j].science,2011,334 (6052): DOI: /science [8] MurphyEF,CoterPD,HealyS,etal.Compositionandenergy harvestingcapacityofthegutmicrobiota:relationshiptodiet,obe sityandtimeinmousemodels[j].gut,2010,59(12): DOI: /gut [9]JakobsdotirG,XuJ,MolinG,etal.High fatdietreducesthe formationofbutyrate,butincreasessuccinate,inflammation,liver fatandcholesterolinrats,whiledietaryfibrecounteractsthese efects[j].plosone,2013,8(11):e80476.doi: / journal.pone [10] TeixeiraTF,GrzeskowiakL,FranceschiniSC,etal.Higherlevel offaecalscfainwomencorelateswithmetabolicsyndromerisk factors[j].brjnutr,2013,109(5): DOI: /S [11] BerggrenAM,NymanEM,LundquistI,etal.Influenceoforaly andrectalyadministeredpropionateoncholesterolandglucoseme tabolisminobeserats[j].brjnutr,1996,76(2): DOI: /BJN [12] ZhengJ,EnrightF,KeenanM,etal.Resistantstarch,fermented resistantstarch,andshort chainfatyacidsreduceintestinalfat depositionincaenorhabditiselegans[j].jagricfoodchem, 2010,58(8): DOI: /jf904583b. [13]RoelofsenH,PriebeMG,VonkRJ.Theinteractionofshort chain fatyacidswithadiposetisue:relevanceforpreventionoftype2 diabetes[j].benefmicrobes,2010,1(4): DOI: /BM ( O@ : )

兽医临床诊断学实验指导

兽医临床诊断学实验指导兽医临床诊断学实验指导河北科技师范学院动物科学系临床兽医学实验室 2009 年 2 月目录学生实验守则... 3 实习一动物的接近保定和基本检查法...4 实习二临床基本检查法及一般检查...10 实习三循环系统的临床检查...15 实习四