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1 温度盐度和密度交互作用对波纹巴非蛤幼贝生长的影响陈志, 高如承, 胡青, 李鹏, 赖素兰 (, ) 摘要 : 为查明波纹巴非蛤 (Paphia undulata) 幼贝最适生长条件, 采用响应面法研究了温度盐度和密度交互作用对波纹巴非蛤幼贝生长的影响利用 Box-Behnken 中心组合进行 3 因素 3 水平的实验设计 (3 因素为温度 (A), 盐度 (B), 密度 (C); 3 水平为 -1, 0, 1), 以幼贝生长率为响应值, 以 A(26, 28, 30 ) B(28, 30, 32) 和 C(0.56, 1.4, 2.24 个 /cm 2 ) 为影响因子实验结果表明 : (1) 盐度对幼贝生长影响最显著 (P<0.05), 3 种生态因子对幼贝生长率影响程度大小顺序是 : B > A > C; (2) 通过 SAS 软件分析, 得出波纹巴非蛤幼贝最适生长环境组合是温度为盐度为密度为 1.12 个 /cm 2, 理论最佳生长率为 41.47% 为验证响应面法预测结果的准确性, 以预测结果为实验条件, 相同实验时间后, 得到的生长率为 42.30%, 结果与理论预测值相近, 响应面方法可靠关键词 : 响应面法 ; 波纹巴非蛤 (Paphia undulata) 幼贝 ; 生长率中图分类号 : P735 文献标识码 : A 文章编号 : (2013) (Paphia undulata) (Mollusca), (Lamellibranchia), (Veneroida), (Veneridae), (Paphia),, 0.5~3.5 m, 10 m [1],,,,, [2], [3-4] [5-6] [7] [8-9] [10] [1] [11-12] (Response Surface Methodolody, : RSM),,, [13-14], [15],,,, [16],,, 1 材料与方法 1.1 实验材料, ,, 100, 4.94 mm±0.07 mm,, 30, 5.04 mm±0.06 mm 1.2 实验设计 1.2.1, 3, Box-Benhnken 3 3 (3 (A), (B), (C); 3 1, 0, 1) : ; : : (2010N5007) ( ) : (1987-),,,, chenzhi200705@163.com; (1958-),,,,,, rcgao@fjnu.edu.cn Marine Sciences / Vol. 37, No. 8 /

2 , 500 ml, 5 mm, ( 32~33) 20 d, (Pyramidomonas sp.),, 1 2 表 1 实验水平和因素设计 Tab. 1 Factors and levels of the experiment A( ) B C( /cm 2 ) 表 2 响应面 3 因素 3 水平实验设计 Tab. 2 The experiment design of 3 factors and 3 levels of RSM A B C , 3,, 20 d, 1.3 计算公式与数据分析 : R g =(L L 0 )/L 0 : R s =(D D 0 )/D 0 L 0, L (: mm), D 0, D SPSS17.0 ANOVA, T, P < 0.05, P < 0.01 SAS 结果 2.1 回归模型和回归方程的建立, SAS 9.2, 3 表 3 响应面分析方案与实验结果 Tab. 3 Experimental result and scheme of RSM R g (%) R s (%) SAS,, (Y) A, B, C : Y A 0.145B 0.237C 0.168A AB 0.170AC 0.262B BC 0.086C 回归方程系数的方差分析和显著性检验 SAS 9.2 Box-Behnken ( 4) 56 / 2013 / 37 / 8

3 表 4 二次多项式模型方差分析和多元回归分析 Tab. 4 Variance analysis of quadratic equation and significance test of regression F Pr F A * B ** C ** AB ** AC BC ** A ** B ** C ** ** ** ** :** P < 0.01; * 0.01< P < 0.05; P > 0.05 ; ; R 2 =0.9848, R 2 adj= , P , 0.01, P ,,,, (P= ) (P= ) (P= ) R 2 adj=0.9574, 95.74%, 4.26% R 2 =0.9848,,,, A, B, C ; AB, BC, AC ; A 2, B 2, C 2, 3 : B A C 2.3 波纹巴非蛤幼贝生长率响应面分析 ( 1) 1 Fig. 1 Response surface plot and contour plot showing the interactive effect of density and temperature on Paphia undulata growth rate Marine Sciences / Vol. 37, No. 8 /

4 ,,,,, ( 2) ( 3),,,, Fig. 2 2 Response surface plot and contour plot showing the interactive effect of salinity and temperature on Paphia undulata growth rate 3 Fig. 3 Response surface plot and contour plot showing the interactive effect of density and silinity on Paphia undulata growth rate SAS9.2, ABC A= 0.534, B= , C= , /cm 2, 41.47% 2.4 模型验证实验结果, 5 ( 6),, 0.83%, 表 5 实验结果验证 Tab. 5 Demonstration of test result L 0 (M±SD)mm L(M±SD)mm R g (%) ±0.81 a 7.81±0.84 b ±0.77 a 7.75±1.06 b ±0.77 a 7.75±1.18 b :, ; P<0.05, P< / 2013 / 37 / 8

5 表 6 验证实验结果与响应面预测结果的对比 Tab. 6 Comparison of the best condition and the predicted condition ( ) ( /cm 2 ) (%) ± ± 讨论,, (Atrina pectinata) [17] (Paphia textzle ) [18] (Scapharca subcrenata) [19],, (Mimachlamys nobilis) [20],,, (Cyclina sinensis) [21],,,,,,,,,,, [22] [23] (Mactra chinensis), (P<0.05) [24] ph (Ruditapes philippinarum),, ph,,,, [25], 40, 55, 30, 5~31, 20~28 [11], 26.93, 42.30%,,,, /cm 2, 41.47%,, 42.30%,, 4 结论, : (1),, (2),,,,, : [1],,,. [J]., 2009, 12: [2],. [J]., 1998, 6(1): [3]. [J]., 2008, 25(3): [4],. (Paphia undulata) [J]., 1992, 11(3): [5]. [J]. :, 2000, 18(3): [6],, 珺. [J]. :, 2002, 20(1): [7],,,. [J]., 2008, 29(11): Marine Sciences / Vol. 37, No. 8 /

6 [8],. [J]., 2010, 7(a): 3. [9],,,. (Paphia undulata) [J]., 2007, 17(1): [10],,. 5 (Paphia undulata) [J]., 2010, 41(1): [11],,,. [J]., 2009, 35(4): [12]. [J]., 2005, 2: [13] Thompson D D. Reponse surface experimentation[j]. Journal of Food Processing and Preservation, 1982, 6(3): [14]. [D]. :, [15],. [J]. ( ), 2005, 14(3): [16]. [M]. :, [17],,. [J]., 2009, 37(5): [18],,. [J]., 2011, 35(10): [19],,,. [J]., 2007, 26(10): [20],,. [J]., 2011, 35(10): [21],,,. [J]., 2006, 30(6): [22],,. [J]., 2009, 10(4): 5-8. [23],,,. [J]., 2009, 24(6): [24],,,. [J]., 2012, 36(9): [25]. (Paphia undulata) [D]. :, The effect of temperature, salinity and density on growth of Paphia undulata youth analyzed by response surface methodology CHEN Zhi, GAO Ru-cheng, HU Qing, LI Peng, LAI Su-lan (College of Life Sciences,Fujian Normal University, Fuzhou , China) Received: Jun.,5,2013 Key words: response surface methodolody; Paphia undulata larvae; growth rate Abstract: In order to explore the optimum growth conditions for Paphia undulata youth, response surface methodology was used to study the effect of temperature, salinity and density on growth of Paphia undulata youth. The experiment was designed according to Box-Behnken central composite design in three factors and three levels, and temperature (26, 28, 30 ), salinity (28, 30, 32), density (0.56,1.4,2.24ind/cm 2 ) were selected as affecting factors. The results showed that the effect of salinity on the growth rate was the most significant (P <0.05), and the order of three ecological factors effects on the youth growth was salinity (B) > temperature (A) > density (C). By means of SAS software, the optimum growth environment condition of Paphia undulata youth was confirmed as follows: temperature 26.93, salinity and density 1.12ind/cm 2, and theoretical optimal growth rate was 41.47%. After experimental verification, the growth rate was 42.30%. To verify the accuracy of the results predicted by RSM, the real growth rate was 42.30% under the theoretical condition, suggesting RSM was reliable. 60 / 2013 / 37 / 8 ( 本文编辑 : 刘珊珊 )

: 307, [], [2],,,, [3] (Response Surface Methodology, RSA),,, [4,5] Design-Expert 6.0,,,, [6] VPJ33 ph 3,, ph, OD, Design-Expert 6.0 Box-Behnken, VPJ3

: 307, [], [2],,,, [3] (Response Surface Methodology, RSA),,, [4,5] Design-Expert 6.0,,,, [6] VPJ33 ph 3,, ph, OD, Design-Expert 6.0 Box-Behnken, VPJ3 微生物学通报 FEB 20, 2008, 35(2) 306~30 Microbiology 2008 by Institute of Microbiology, CAS tongbao@im.ac.cn 生物实验室响应面分析法优化副溶血性弧菌生长条件刘代新宁喜斌 * 张继伦 2 (. 200090) (2. 20202) 摘要